The MoleStrips DNA Tissue Kit is used together with the GeneMole instrument to purify genomic DNA from solid tissue samples. The following kit formats are available:
MG11-101/731-3052: Convenience kit containing 32 MoleStrips and all required tips,tubes and caps. This kit supports isolation of genomic DNA from 32 different tissue samples.
MG11-102/731-3054: Kit containing 64 MoleStrips supporting isolation of genomic DNA from 64 different tissue samples. Tips, tubes and caps can be ordered from VWR.
One MoleStrip contains all the reagents needed to purify genomic DNA from one tissue sample including magnetic beads in binding buffer, two different washing buffers, 70 % EtOH and elution buffer. The MoleStrips are sealed and the instrument pierces holes in the foil during the run.
Starting Sample
The MoleStrips DNA Tissue Kit has been tested for mouse muscle, liver, spleen, hart, lung, ear, tail, kidney and brain. The tissue samples are lysed in lysis buffer and Proteinase K overnight before the lysate is placed in the GeneMole and the automated DNA extraction can start
Yield and Purity
Different tissues have different DNA content meaning that the DNA yield will depend on what sort of tissue is used. Average yield from 10 mg mouse samples was in the range 20 µg/ml (for muscle) to 75 µg/ml (for liver). DNA purity, measured as OD A260 /A280, was in the range 1.80-2.00. Isolated genomic DNA is suitable for sensitive downstream applications
# 02 DNA extraction from mouse tissue
